目的 :从菘蓝的同源四倍体植物诱导出毛状根 ,并探讨用毛状根代替自然根作为药用的可能性。 方法 :利用发根农杆菌 A4菌株感染四倍体菘蓝 ,采用细菌内毒素检查法考察诱导所得毛状根的抗内毒素作用。结果 :A4菌株能诱导四倍体菘蓝产生毛状根 ,毛状根具有较强的抗内毒素作用。 结论 :发根农杆菌诱导四倍体植物产生的毛状根有可能成为传统中药材的一种代用品。 Objective : To induce hairy roots from indigo-autotetraploid plants and to explore the possibility of using hairy roots instead of natural roots for medicinal purposes. Methods: The tetraploid indigo was infected by Agrobacterium rhizogenes strain A4. The bacterial endotoxin test was used to examine the anti-endotoxin effects of the induced hairy roots. Results : A4 strain could induce hairy roots of tetraploid indigo, and hairy roots had strong antiendotoxin effect. Conclusion : The hairy roots produced by Agrobacterium rhizogenes to induce tetraploid plants may become a substitute for traditional Chinese medicines.