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目的探讨砷致肝损伤大鼠体内砷含量和肝功能受竹荪多糖干预的影响。方法 108只成年清洁级SD大鼠随机分为正常对照组(24只,以普通饲料喂饲)、砷+竹荪同时干预组[(简称同时干预组),24只,10 mg/ml的竹荪多糖每日20ml/kg灌胃,且饲料中砷含量为50 mg/kg]、砷染毒组(60只,喂饲砷含量50 mg/kg的饲料),均雌雄各半。采用喂饲法进行染毒3个月后,以HE、Masson染色观察肝损伤情况。继续将砷染毒组随机均分为砷染毒组、砷+二巯基丙磺酸钠(DMPS)组[(简称DMPS组),每天以5 mg/kg二巯基丙磺酸钠腹腔注射,连续3 d,间隔4 d为一个周期]、砷+竹荪后干预组[(简称竹荪后干预组),10 mg/ml竹荪多糖每日20 ml/kg灌胃],每组18只,雌雄各半,三组均以砷含量为50 mg/kg的饲料喂饲,各组再处理3个月,观察大鼠体内砷含量和肝功能的变化情况。结果肝脏HE、Masson染色显示,与正常对照组比较,同时干预组、砷染毒组光镜下肝组织均有不同程度肝损伤出现。与正常对照组相比,各处理组大鼠血清ALT和AST的活力升高,且随着染毒时间的增加,各组大鼠血清ALT和AST的活力呈上升趋势。与正常对照组相比,各处理组大鼠血、尿、肝脏砷含量均增高(P<0.05)。与砷染毒组相比,同时干预组、DMPS组、竹荪后干预组肝砷降低,血砷和尿砷升高(P<0.05)。与DMPS组相比,同时干预组肝砷先降低后升高,尿砷先升高后减低,血砷先升高后减低再升高(P<0.05)。与同时干预组相比,竹荪后干预组肝砷升高,血砷、尿砷降低(P<0.05)。与竹荪后干预组相比,DMPS组肝砷降低,尿砷升高,血砷先升高后降低(P<0.05)。结论竹荪多糖早期干预能减轻砷中毒大鼠肝损伤程度,且竹荪在砷中毒早期干预对肝损伤的减轻效果优于砷中毒后再干预,但竹荪多糖对砷中毒大鼠体内驱砷效果仍不及二巯基丙磺酸钠。
Objective To investigate the effects of arsenic-induced liver injury on the levels of arsenic and hepatic function in polysaccharides of Dodophos. Methods One hundred and eight adult Sprague-Dawley rats were randomly divided into normal control group (24 fed normal diet), arsenic + Dictyophora simultaneous intervention group (abbreviated as simultaneous intervention group), 24, 10 mg / ml of bamboo (20 mg / kg daily, and the arsenic content in the diet was 50 mg / kg), and the arsenic-exposed group (60 pigs fed the arsenic content 50 mg / kg). After 3 months of feeding, the liver damage was observed by HE and Masson staining. The arsenic-exposed group was continued to be randomly divided into arsenic-exposed group, arsenic + sodium dimercaptopropane sulfonate (DMPS) group (DMPS group), intraperitoneal injection of 5 mg / kg sodium dimercaptopropane sulfonate daily for continuous 3 d, 4 d intervals for one cycle], arsenic + Dictyophora posterior intervention group [(referred to as Dictyophora after intervention group), 10 mg / ml Dictyophora polysaccharide 20 ml / kg orally], each group 18, The male and the female were fed with the arsenic content of 50 mg / kg. The animals in each group were treated for another 3 months. The changes of arsenic and liver function in rats were observed. Results Hepatic HE and Masson staining showed that compared with the normal control group, hepatic injury occurred in liver tissue of the intervention group and the arsenic-exposed group under different light microscope. Compared with the normal control group, the activities of serum ALT and AST increased in all treatment groups, and the activities of serum ALT and AST increased with the increase of exposure time. Compared with the normal control group, the arsenic levels of blood, urine and liver were increased in all treatment groups (P <0.05). Compared with the arsenic-exposed group, the arsenic and arsenic concentrations in the intervention group, the DMPS group and the Dictyophora group were lower than those in the arsenic-exposed group (P <0.05). Compared with the DMPS group, hepatic arsenic decreased first and then increased in the intervention group, while the urinary arsenite increased first and then decreased, while the blood arsenic increased first and then decreased (P <0.05). Compared with the same intervention group, Dictyophora posterior intervention group increased liver arsenic, blood arsenic, urinary arsenic decreased (P <0.05). Compared with the Dictyophora group, the arsenic and the arsenite in the DMPS group increased and the blood arsenic increased first and then decreased (P <0.05). Conclusion Dictyoshnophyllum polysaccharide early intervention can reduce arsenic poisoning in rats liver damage, and Dictyophora arsenic poisoning early intervention on liver damage mitigation effect is better than arsenic poisoning before intervention, but Dictyophora polysaccharide on arsenic poisoning rat arsenic poisoning The effect is still less than sodium dimercaptopropane sulfonate.