目的:运用指纹图谱技术,对不同采收月份、采收时间和不同加工方法的凉粉草进行质量评价,确定最佳采收时间和适宜的加工方法。方法:采用高效液相色谱法测定,色谱柱为Dikma Diamonsil(2)C18柱(250 mm×4.6 mm,5μm);流动相为乙腈-2%甲酸(梯度洗脱);检测波长为320 nm;流速为0.8 ml.min-1;柱温为25℃。结果:凉粉草最佳采收月份为9～10月,最佳采收时间为午后16:00左右;加工方法可根据用途作相应的加工。结论:该法准确可靠,重复性好,为凉粉草药材生产质量控制提供了依据。 OBJECTIVE: To evaluate the quality of jelly grass with different harvesting months, harvesting time and different processing methods by fingerprinting, and to determine the optimal harvesting time and suitable processing methods. Methods: The chromatographic column was Dikma Diamonsil (2) C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-2% formic acid (gradient elution). The detection wavelength was 320 nm. The flow rate was 0.8 ml.min-1; the column temperature was 25 ° C. Results: The best harvesting season of Grifola frondosa was from September to October, and the best harvesting time was around 16:00 in the afternoon. The processing method could be processed according to the purpose. Conclusion: The method is accurate and reproducible, and provides a basis for quality control of jelly grass medicinal materials.