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目的:观察姬松茸多糖(Agaricus blazei polysaccharides,ABP)的抗过敏作用并探讨其可能的作用机制。方法:通过IgE诱导大鼠被动皮肤过敏反应(PCA)实验,用比色法测定姬松茸多糖在体内对肥大细胞影响。体外实验观察姬松茸多糖对IgE诱导组胺释放、肿瘤坏死因子-α、白细胞介素6及p38MAPK的影响。结果:体内实验显示,姬松茸多糖高(400mg/kg)、中(200mg/kg)剂量明显抑制大鼠PCA。姬松茸多糖高(100μg/L)、中(50μg/L)剂量了肥大细胞脱颗粒、组胺的释放、以及肿瘤坏死因子-α、白细胞介素6的释放以及p38MAPK、NF-κB p65蛋白的表达。结论:姬松茸多糖抗过敏作用部分可能与抑制p38MAPK、NF-κB p65信号通路有关。
Objective: To observe the anti-allergic effect of Agaricus blazei polysaccharides (ABP) and to explore its possible mechanism. Methods: Passive skin allergic reaction (PCA) was induced by IgE in rats. The effect of Agaricus blazei Murill polysaccharide on mast cells was measured by colorimetry. Effect of Agaricus Blazei Murill polysaccharide on IgE-induced Histamine Release, Tumor Necrosis Factor-α, Interleukin-6 and p38MAPK in Vitro Experiment. Results: In vivo experiments showed that the high dose of Agaricus blazei polysaccharides (400mg / kg) and medium dose (200mg / kg) significantly inhibited PCA in rats. Agaricus blazei polysaccharides (100μg / L), medium (50μg / L) dose of mast cell degranulation, histamine release, and the release of tumor necrosis factor-α, interleukin 6 and p38MAPK, NF-κB p65 protein expression. Conclusion: The anti-allergic effect of Agaricus blazei polysaccharides may be related to the inhibition of p38MAPK and NF-κB p65 signaling pathway.