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目的:通过球囊损伤犬颈动脉模型观察曲尼司特对颈动脉损伤后再狭窄的作用。方法:9只犬被随机分为对照组(n=5)及曲尼司特干预组(50mg·kg-1,n=4),颈总动脉损伤前2周及术后4周进行分组干预。通过测定血浆AngⅠ及AngⅡ水平、糜酶(chymase)mRNA表达水平、颈动脉细胞增殖核抗原(PCNA)阳性率及颈动脉各层厚度观察曲尼司特对犬颈动脉损伤后狭窄的作用。结果:两组血浆AngⅠ、AngⅡ水平及颈动脉外/中膜厚度无明显改变(P>0.05);但曲尼司特组较对照组的糜酶mRNA表达(A值分别为0.425±0.114比0.708±0.083)、颈动脉各层PCNA阳性率(内膜:0.45±0.05比0.57±0.12,中膜:0.54±0.05比0.61±0.02,外膜:0.25±0.10比0.36±0.08)及颈动脉内/中膜厚度(0.518±0.044比0.576±0.028)均明显降低,P<0.05。结论:曲尼司特通过糜酶途径对犬颈动脉球囊损伤后的再狭窄具有抑制作用。
OBJECTIVE: To observe the effects of tranilast on the restenosis after carotid artery injury by means of balloon injury of carotid artery in dogs. Methods Nine dogs were randomly divided into control group (n = 5) and tranilast intervention group (50 mg · kg-1, n = 4), two weeks before and two weeks after carotid artery injury . The effect of tranilast on the stenosis of carotid artery in dogs was observed by measuring the plasma levels of AngⅠ and AngⅡ, the expression of chymase mRNA, the positive rate of carotid artery proliferating cell nuclear antigen (PCNA) and the thickness of carotid artery. Results: There were no significant changes in plasma AngⅠand AngⅡ levels and carotid artery / medial thickness in both groups (P> 0.05). However, the mRNA expression of chymase in the tranilast group was significantly higher than that of the control group (A values were 0.425 ± 0.114 vs 0.708 ± 0.083). The positive rate of PCNA in the carotid arteries (intima: 0.45 ± 0.05 vs 0.57 ± 0.12, in the media: 0.54 ± 0.05 vs 0.61 ± 0.02, adventitia: 0.25 ± 0.10 vs 0.36 ± 0.08) Median thickness (0.518 ± 0.044 vs 0.576 ± 0.028) were significantly lower, P <0.05. CONCLUSION: Tranilast inhibits the restenosis after carotid artery balloon injury in dogs through chymase pathway.