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42条成年杂种犬(体重25～30kg)在静脉麻醉和控制呼吸下开胸,左前降支(LAD)近端插管并通过压缩泵连接于右股动脉。首先调整血流量,使LAD灌注压等于平均主动脉压;在随后实验过程中,血流量保持不变。实验在切断颈迷走神经、切断星状神经节与中枢的联接纤维并阻断β受体(静脉注入普荼洛尔1.0mg/kg,随后0.5mg/hr)的条件下
Forty-two adult canines, weighing 25-30 kg, underwent thoracotomy under venous anesthesia and controlled breathing, were cannulated proximal to the left anterior descending (LAD) and connected to the right femoral artery via a compression pump. The blood flow was adjusted first to equal LAD perfusion pressure to mean aortic pressure; blood flow remained unchanged during the subsequent experiments. The experiment was performed under the condition of cutting off the cervical vagus nerve, cutting off the connecting fibers between the stellate ganglion and the center and blocking the β receptor (intravenous injection of preslopodiaol 1.0mg / kg, then 0.5mg / hr)