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目的了解天津市HIV-1毒株的流行情况,比较不同感染途径HIV-1感染者中流行毒株的亚型分布。方法采集100例感染途径已知的HIV-1感染者抗凝全血标本,提取DNA,用巢式聚合酶链式反应扩增病毒gag基因,并进行序列测定和亚型分析。结果 100份样品中,有87份样品被成功扩增出HIV-1的gag基因片段,通过系统进化分析,确定天津市HIV-1流行毒株分属4个亚型和重组型,其中CRF01_AE比例最大,达59.77%(52/87),主要分布在通过同性和异性性传播的HIV-1感染者中,而在通过静注吸毒感染的HIV-1感染者中以CRF07_BC亚型为主。结论天津市HIV-1流行株至少有4种基因亚型,且不同感染途径HIV-1感染者流行毒株的亚型分布略有不同,应加强对HIV-1毒株亚型变异的监测,及时调整防治策略。
Objective To understand the prevalence of HIV-1 strains in Tianjin and to compare the distribution of subtypes of HIV-1 strains among HIV-1 infected persons with different routes of infection. Methods 100 anticoagulant whole blood samples from HIV-1 infected patients were collected and DNA was extracted. The viral gag gene was amplified by nested polymerase chain reaction and sequenced and subtype analyzed. Results Among 100 samples, 87 samples were successfully amplified gag gene fragment of HIV-1. Through phylogenetic analysis, HIV-1 strains in Tianjin belonged to 4 subtypes and recombinant types, of which CRF01_AE ratio (59.77%, 52/87), which were mainly distributed in HIV-1 infected persons who were transmitted by homosexual and heterosexual transmission. However, CRF07_BC was predominant in HIV-1 infected by intravenous injection. Conclusion There are at least 4 subtypes of HIV-1 strains in Tianjin and subtypes of HIV-1 infected persons are slightly different in different routes of infection. Surveillance of subtypes of HIV-1 strains should be strengthened, Timely adjustment and control strategy.