目的 :从人血浆中分离纯化载脂蛋白H(apolipoproteinH ,apoH) ,经免疫家兔 ,获得特异性抗血清 ,采用Iodogen法进行12 5I标记apoH ,建立了人血清apoH放射免疫分析 (RIA)方法。结果 :方法灵敏度为2 33mg/L ,工作范围 5～ 32 0mg/L ,批内和批间CV分别为 4 93和 4 0 1% ,回收率为 95 7% ,与apo -AI、apo-B未见交叉反应。测定了 91例正常人血清apoH含量 ,正常值 :男性为 2 30 17± 5 6 4mg/L ,女性为 2 0 0 2 7± 4 2 5mg/L ,男女两组之间血清apoH水平差异无显著性。 Objective: To isolate and purify apolipoprotein H (apoH) from human plasma and obtain specific antiserum by immunizing rabbits. ApoH radiolabeled immunoassay (RIA) was established by 125 I-labeled apoH using Iodogen method . Results: The sensitivity of the method was 233 mg / L, the working range was 5 ~ 32 0 mg / L, the intra-and inter-assay CV was 493% and 41% respectively. The recovery rate was 95.7% No cross-reaction. Serum apoH levels were measured in 91 normal subjects. The normal values ​​were 2 30 17 ± 56 4 mg / L for males and 2 0 0 2 7 ± 4 2 5 mg / L for females. There was no significant difference in serum apoH levels between men and women Sex.