论文部分内容阅读
目的：研究丙型肝炎病毒Ｃ３３ｃ抗原及核心抗原在人肝内胆管细胞癌中的分布及意义。方法：以免疫组织化学方法检测ＨＣＶ两种抗原及ＨＢｘＡｇ在肝内胆管细胞癌及癌旁肝组织中定位及分布。结果：ＨＣＶＣ３３ｃ抗原位于癌细胞或肝细胞的胞浆内；ＨＣＶ核心抗原在癌组织主要呈核阳性，在癌旁肝以胞浆阳性为主。ＨＣＶＣ３３ｃ抗原在癌组织及癌旁肝的阳性率分别为６０％（２１／３５）及１００％（２１／２１），ＨＣＶ核心抗原在上述两种组织的阳性率分别为８７．８％（２９／３３）及６１．９％（１３／２１）；ＨＢｘＡｇ在两种组织中检出率分别为７４．３％（２６／３５）及７６．２％（１６／２１）．Ｃ３３ｃ及ＨＢｘＡｇ双阳性分别为４８．６％（１７／３５）及７６．２％（１６／２１）．结论：除ＨＢＶ感染外，ＨＣＶ感染在人肝内胆管细胞癌的发生中可能起重要作用
Objective: To study the distribution and significance of hepatitis C virus C33c antigen and core antigen in human intrahepatic cholangiocarcinoma. Methods: Immunohistochemistry was used to detect the localization and distribution of HCV two antigens and HBxAg in intrahepatic cholangiocarcinoma and adjacent liver tissues. RESULTS: HCVC33c antigen was located in the cytoplasm of cancer cells or hepatocytes; HCV core antigen was mainly nuclear positive in cancer tissues, and cytoplasm positive in the paraneoplastic liver. The positive rates of HCVC33c antigen in cancer tissue and adjacent liver were 60% (21/35) and 100% (21/21), respectively. The positive rate of HCV core antigen in the above two tissues was 87.8% (29/ 33) and 61.9% (13/21); The detection rates of HBxAg in the two tissues were 74.3% (26/35) and 76.2% (16/21), respectively. The positive rates of C33c and HBxAg were 48.6% (17/35) and 76.2% (16/21), respectively. Conclusion: In addition to HBV infection, HCV infection may play an important role in the development of human intrahepatic cholangiocarcinoma