在植物电镜样品的超薄切片中,材料的定位很重要,特别是对那些用肉眼无法辨别的微小材料如胚囊及其内部的卵器、组织培养中的胚性细胞等则更困难。Kennell(1985)曾介绍一种重包埋技术,但该技术步骤多,需要时间长,操作较复杂。我们参考了Kennell的工作,对重包埋技术作了较大改进,解决了微小材料定位难的问题。 In ultrathin sections of SEM samples, the positioning of the material is important, especially for tiny materials that are indistinct to the naked eye, such as embryo sacs and their internal argillaceous cells, embryogenic cells in tissue culture, and the like. Kennell (1985) introduced a heavy embedding technique, but this technique requires more steps and takes more complicated steps. With reference to Kennell’s work, we greatly improved the heavy embedding technique to solve the problem of difficult positioning of small materials.