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本文报道了高效液相色谱法测定口腔溃疡散靛蓝的含量。用氯仿提取 ,以甲醇—水—冰乙酸 ( 75:2 5:0 .0 2 )为流动相。流速 1m/ min,检测波长 2 85nm,平均回收率为 :98.71%。 RSD为 :1.2 3%。
This article reported the HPLC method for the determination of oral ulcer disinhibitor blue content. Extract with chloroform and use methanol-water-glacial acetic acid (75:25:0.02) as the mobile phase. Flow rate 1m/min, detection wavelength 285nm, average recovery rate: 98.71%. The RSD is :1.2 3%.