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目的了解郧县家禽屠宰点从业人员禽流感H5N1感染及外环境禽流感病毒H5、H9亚型污染状况。方法 1月份采集家禽屠宰点从业人员16人血清标本用红细胞凝集抑制试验(HI)检测H5N1抗体;1-12月每月各采集5份外环境鸡笼、鸡粪、污水标本用荧光定量PCR法(Real-time PCR)检测禽流感病毒FluA、AH5、AH9、AN1核酸。结果 16份家禽屠宰点从业人员血清H5N1抗体均为阴性;家禽屠宰点60份外环境标本中,阳性检出(率)分别为FluA 30份(50.00%)、AH9 19份(31.67%)、AH5 1份(1.67%)、AN1 1份(1.67%);30份FluA阳性标本中,仅AH9阳性18份,构成比为60.00%;AH9、AH5全为阴性11份,构成比为36.67%;AH9、AH5同时阳性1份,构成比为3.33%;FluA、AH9、AH5、AN1在11月同一份污水标本中检出。结论郧县家禽屠宰点外环境存在禽流感病毒AH9、AH5等多种亚型污染,且存在H5N1的污染,但没有从业人员感染H5N1的证据。
Objective To understand the H5N1 infection of H5N1 and H5, H9 subtype of avian influenza virus in poultry slaughterhouse in Yun County. Methods In January, 16 serum samples collected from poultry slaughterhouses were used to detect H5N1 antibody by hemagglutination inhibition test (HI). From January to December each month, 5 samples of external environment cages, chicken manure and sewage samples were collected by fluorescence quantitative PCR (Real-time PCR) detection of FluA, AH5, AH9, AN1 nucleic acids. Results Sixteen serum samples from slaughterhouses were negative for H5N1 antibody. Among the 60 samples of slaughterhouses, the positive detection rates were 30 (50.00%) FluA, 19 (31.67%) AH9, and AH5 AHA and AH5 were all negative, with the proportion of 36.67%; AH9, AH5 were all negative, the proportion of AH9 was 15.6% , AH5 positive at the same time 1, the constituent ratio was 3.33%; FluA, AH9, AH5, AN1 detected in the same sewage samples in November. Conclusion There are many subtypes of avian influenza virus AH9 and AH5 in the external environment of poultry slaughter point in Yun County. There is also contamination of H5N1 but no evidence of H5N1 infection by practitioners.