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本研究以东乡野生稻耐冷渐渗系IL5243和IL5335及其双亲为试验材料,利用139对SSR引物和35对AFLP引物分析东乡野生稻DNA在两耐冷渐渗系中的遗传与DNA序列变化特征,探讨基因组结构和序列变化对外源基因渐渗的作用及影响。SSR和AFLP的分析结果表明,BC1F10的耐冷渐渗系对双亲基因组的继承趋于稳定、纯合,绝大多数条带(85.15%~90.77%)与受体亲本协青早B一致,4.33%~7.17%的东乡野生稻外源DNA条带已成功导入渐渗系中。DNA测序及BLAST序列比对结果表明,被导入的外源DNA序列具有抗逆、抗病或其他生物学功能,但两耐冷渐渗系在遗传继承双亲遗传物质的同时,还呈现一些基于基因组DNA序列变化的非孟德尔式变异,包括亲本序列丢失和新序列出现以及碱基的颠换或转换,这些序列变异不仅可发生在非编码区的重复序列中,也可发生在功能基因序列中。研究结果表明东乡野生稻DNA渐渗诱发受体基因组产生广泛的变异,对拓宽栽培种遗传变异具有重要意义。
In this study, we used 139 pairs of SSR primers and 35 pairs of AFLP primers to analyze the genetic variation and DNA sequence of Dongxiang wild rice in two tolerant introgression lines, using IL5243 and IL5335, To investigate the role and influence of genomic structure and sequence changes on introgression of exogenous genes. The results of SSR and AFLP analysis showed that inheritance of cold tolerance and introgression lines of BC1F10 to the parental genome tended to be stable and homozygous. The vast majority of bands (85.15% ~ 90.77%) were consistent with the recipient parent Xieqingzao B, with 4.33% ~ 7.17% of Dongxiang wild rice exogenous DNA bands have been successfully introduced into the introgressing system. DNA sequencing and BLAST sequence alignment showed that the foreign DNA sequences introduced had anti-retrogradation, disease resistance or other biological functions. However, while the two resistance-tolerant introgression lines inherit inheritance of their parents’ genetic material, some DNA-based sequencing based on genomic DNA Non-Mendelian variations in sequence variation, including loss of parent sequence and appearance of new sequences, and transversion or conversion of bases, can occur not only in repeat sequences in non-coding regions but also in functional gene sequences. The results showed that DNA introgression of Dongxiang wild rice induced extensive variation of the genome of the recipient, which is of great significance to broaden the genetic variation of cultivated species.