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目的 :检测IL 6对原代白血病细胞的影响 ,探讨其在地塞米松耐药机制中的作用。方法 :①不同条件下体外培养原代白血病细胞 ,用MTT法了解细胞增殖情况 ,②用形态学观察和原位末端标记法检测不同培养条件下HL 6 0细胞株的细胞凋亡。结果 :①IL 6可显著促进AML细胞增殖 (P <0 .0 5 ) ,对ALL细胞则无影响 (P >0 .0 5 ) ,②地塞米松显著抑制AML细胞增殖 (P <0 .0 5 ) ,且这种抑制在部分病例可被IL 6逆转 (P >0 .0 5 ) ,③地塞松米可诱导HL 6 0细胞凋亡 ,凋亡率为 13.0 6 %。结论 :①地塞米松抑制AML细胞的增殖是诱导白血病细胞发生凋亡 ;②IL 6在急性白血病发病及地塞米松耐药中可能发挥着调控作用
Objective: To detect the effect of IL 6 on primary leukemia cells and to explore its role in the mechanism of drug resistance in dexamethasone. Methods: ①The primary leukemic cells were cultured in vitro under different conditions, MTT assay was used to investigate the cell proliferation. ② The apoptosis of HL 60 cell line was detected by morphological observation and in situ end labeling. Results: ① IL 6 significantly promoted the proliferation of AML cells (P <0.05), but had no effect on ALL cells (P> 0.05). ② Dexamethasone significantly inhibited the proliferation of AML cells (P <0.05) ), And this inhibition could be reversed by IL-6 (P> 0.05) in some cases. (3) Dexsumustine could induce the apoptosis of HL-60 cells with the apoptosis rate of 13.0 6%. Conclusion: (1) Dexamethasone inhibits the proliferation of AML cells and induces the apoptosis of leukemic cells; (2) IL 6 may play a regulatory role in the pathogenesis of acute leukemia and dexamethasone resistance