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为探讨一种非侵入性妊娠早期产前诊断方法的实用性,选择30例妊娠6～10周的孕妇作为研究组,应用非侵入技术——子宫颈刷取法以获得胎儿细胞并结合荧光原位杂交(FISH)方法,进行胚胎性别的诊断,研究组均按计划终止妊娠,取其绒毛组织进行染色体核型分析,以行对照。 方法 标本采集:用巴氏细胞涂片刷,自宫颈外口向内插入<2 cm经充分旋转后取出,分别在4个载玻片(接顺序编号)上涂片、固定、风干后备检。取材后的孕妇行吸宫术,取绒毛组织送实验室培养。试验步骤:原位杂交;涂片后的载玻片
To explore the utility of a noninvasive method of prenatal diagnosis of early pregnancy, 30 pregnant women of 6 to 10 weeks of gestation were selected as the study group. Non-invasive technique - cervical brushing was used to obtain fetal cells combined with fluorescence in situ Hybridization (FISH) method for the diagnosis of embryonic sex, the study group were terminated according to plan pregnancy, taking their chorionic karyotype analysis, to control. Methods Specimen collection: with Pap smear brush, insert the <2 cm inward from the cervix into the full rotation after removal, were slides in four slides (serial number), fixed, air dried for the next seizure. Draw after the pregnant women aspiration of the uterus, take villus tissue sent to the laboratory culture. Test procedure: in situ hybridization; slides after smear