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对拮抗细菌YD4-6和NV11-4防治辣椒疫病的作用机制进行了初步研究。通过在辣椒苗上接种辣椒疫霉菌和2株拮抗细菌,测定PPO、POD、PAL、SOD的活性及MDA含量变化。结果表明,接种NV11-4菌液处理24h后4种防御酶活性均升高,但在YD4-6菌液处理中,PPO和SOD活性变化基本接近对照水平,而经NV11-4和YD4-6处理的辣椒叶片内POD活性分别在24 h和72 h达到最大值,其峰值为12.44和12.89;两者处理的PAL活性均于接种后48 h达到最大值,其中前者比后者高1.22倍;MDA含量与对照相比无明显变化,表明2株拮抗菌对辣椒植株没有伤害。
The mechanism of antagonistic bacteria YD4-6 and NV11-4 against pepper blight was studied. The activity of PPO, POD, PAL and SOD and the content of MDA were determined by inoculation of Phytophthora capsici and two antagonistic bacteria on pepper seedlings. The results showed that the activities of four defense enzymes increased after inoculation of NV11-4 bacterium liquid for 24 h, but in the treatment of YD4-6, the changes of PPO and SOD activities were basically close to the control level, while the NV11-4 and YD4-6 The activities of POD in the leaves of peppers reached the maximum at 24 h and 72 h, respectively, with the peak values of 12.44 and 12.89, respectively. The PAL activity of the two treatments reached the maximum at 48 h after inoculation, of which the former was 1.22 times higher than the latter. MDA content compared with the control did not change significantly, indicating that two strains of antagonistic bacteria pepper plants without damage.