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以长期不同施肥处理土壤为研究对象,运用荧光素二乙酸酯(FDA)为底物,采用分光光度法测定土壤FDA水解酶活性.结果表明:在黑土和棕壤上有机肥配施化肥处理的FDA水解酶活性增幅较大,相对于不施肥处理分别增加68.8%和72.4%,也显著高于单施化肥处理;土壤FDA水解酶活性与土壤微生物量碳具有极显著的正相关关系,在黑土和棕壤中相关系数分别为0.912**和0.871**;FDA水解酶活性能较好地反映微生物活性,可以作为评价土壤微生物量和活性的重要指标;同时,土壤FDA水解酶与参与C、N、P、S等重要养分循环的酶活性关系密切,均达到极显著水平.
Taking long-term different fertilization treatments as the research object, the FDA hydrolase activity was determined by spectrophotometry using fluorescein diacetate (FDA) as the substrate.The results showed that the application of chemical fertilizers on black soil and brown soil The activities of FDA hydrolase increased significantly, which were increased by 68.8% and 72.4% compared with those without fertilization, respectively. The activities of FDA hydrolase in soil were significantly positively correlated with soil microbial biomass carbon The correlation coefficients of black soil and brown soil were 0.912 ** and 0.871 **, respectively. The activity of FDA hydrolytic enzyme could better reflect the activity of microorganism, which could be used as an important index to evaluate soil microbial biomass and activity. At the same time, , N, P, S and other important nutrient cycling enzyme activity are close, all reached a significant level.