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消化管神经丛的分布及其生理活动的研究甚多,但对低等脊椎动物的研究报道较少.扬子鳄是我国特有的世界珍稀动物,本文用Karnovsky-Roots乙酰胆碱酯酸(AChE)组化法研究扬子鳄消化管粘膜下神经丛的分布,为肠神经丛的比较解剖学研究提供一些形态学资料.1 材料和方法 扬子鳄2条(雌雄各1),2%戊巴比妥钠10～15ml腔内注射麻醉,打开胸腹腔,从左心室灌注生理盐水,继而灌流4%多聚甲醛固定,取食管下段、胃体、十二指肠、空肠、回肠和大肠,置4%多聚甲醛固定6h,继入合20%蔗糖的0.1mol·L~(-1)PB液中,4℃过夜.制备厚25μm的冷冻切片.以亚铁氰化铜法显示AChE,孵育液内加四异丙基焦磷酰胺(iso-OMPA)非特异性胆碱酯酶抑制剂和免去底物为对照.
There are many studies on the distribution and physiological activities of the digestive tract plexus, but few reports on lower vertebrates. Alligator sinensis is rare in the world. In this study, Karnovsky-Roots acetylcholinesterase (AChE) To study the distribution of submucosal nerve plexus in the digestive tract of Alligator sinensis and to provide some morphological data for the comparative anatomy of the intestinal nerve plexus.Materials and Methods 2 Alligators (1 male and 1 female), 2% Pentobarbital 10 ~ 15ml Intrathecal anesthesia, open the chest and abdomen cavity, from the left ventricular perfusion saline, and then perfusion 4% paraformaldehyde fixed, take the lower esophagus, corpus, duodenum, jejunum, ileum and colon, set 4% paraformaldehyde fixed 6h , Followed by the addition of 20% sucrose in 0.1 mol·L -1 PB solution overnight at 4 ° C. Frozen sections with a thickness of 25 μm were prepared, and AChE was shown by the method of ferrocyanide, and tetraisopropyl The iso-OMPA nonspecific cholinesterase inhibitor and substrate-free control served as controls.