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将小鼠白细胞介素３（ＩＬ－３）ｃＤＮＡ重组到逆转录病毒载体ｐＮ２Ａ质粒上，用电穿孔法将此重组子转染包装细胞系（ＰＡ３１７），经用Ｇ４１８（ｇｅｎｅｔｉｃｉｎ）选择培养基筛选，得到产病毒效价为每ｍｌ５×１０４～２×１０５ＣＦＵ（ｃｏｌｏｎｙ－ｆｏｒｍｉｎｇｕｎｉｔ）的Ｇ４１８抗性细胞克隆。然后取其培养上清液转染ＮＩＨ／３Ｔ３细胞。通过检测被病毒转染的ＮＩＨ／３Ｔ３细胞培养上清液对小鼠骨髓细胞的增殖反应（ＭＴＴ法）和促分化作用（ＣＦＵ培养法），表明转染的ＮＩＨ／３Ｔ３细胞克隆能分泌ＩＬ－３。此结果为进行ＩＬ－３转基因治疗研究奠定了实验基础。
The murine interleukin 3 (IL-3) cDNA was recombined into the retroviral vector pN2A plasmid, and the recombinant was transfected into the packaging cell line (PA317) by electroporation and was screened with G418 (geneticin) selection medium To obtain a G418 resistant cell clone with virus titer of 5 × 104 to 2 × 10 5 CFU per ml (colony-forming unit). Then take the culture supernatant transfected NIH / 3T3 cells. The proliferation of mouse bone marrow cells (MTT assay) and differentiation (CFU culture) assay of NIH / 3T3 cell culture supernatant transfected with the virus indicated that the transfected NIH / 3T3 cell clones secreted IL- 3. This result laid the experimental foundation for the study of IL-3 transgenic treatment.