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目的建立同时测定珍珠菜提取物中芦丁、异鼠李素-3-O-芸香糖苷、江户樱花苷、槲皮素、山柰酚的HPLC方法。方法采用Chromasil C18色谱柱(250 mm×4.6 mm,5.0μm),柱温35℃,以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,双波长检测(λ1=283 nm,λ2=370 nm),体积流量1.0 mL/min。结果 5个成分均能达到基线分离,线性回归方程分别为芦丁Y=14 958 X+179.22(r=0.999 3),异鼠李素-3-O-芸香糖苷Y=12 126 X+3.14(r=0.999 4),江户樱花苷Y=23 821 X+76.81(r=0.999 4),槲皮素Y=35 761 X-20.30(r=0.999 5),山柰酚Y=39 078 X+1.81(r=0.999 1),芦丁、异鼠李素-3-O-芸香糖苷、江户樱花苷、槲皮素、山柰酚进样量分别在228.60～1 143.00、99.60～498.00、232.20～1 161.00、22.08～110.40、15.12～75.60 ng与峰面积线性关系良好,平均回收率分别为97.8%、98.9%、102.4%、98.4%、92.2%。结论本检测方法简便、准确,为珍珠菜提取物中黄酮类成分的质量控制提供了依据。
OBJECTIVE To establish a HPLC method for the simultaneous determination of rutin, isorhamnetin-3-O-rutinoside, vinca saponin, quercetin and kaempferol in the extract of pearl. Methods Chromasil C18 column (250 mm × 4.6 mm, 5.0 μm) was used. The column temperature was 35 ℃. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution with gradient elution and dual wavelength detection (λ1 = 283 nm, λ2 = 370 nm ), Volume flow 1.0 mL / min. Results The five components all achieved baseline separation. The linear regression equations were rutin Y = 14 958 X + 179.22 (r = 0.999 3), isorhamnetin-3-O-rutinoside Y = 12 126 X + 3.14 r = 0.999 4), Edomatin Y = 23 821 X + 76.81 (r = 0.999 4), quercetin Y = 35761 X-20.30 (r = 0.999 5), and kaempferol Y = 39 078 X + 1.81 (r = 0.999 1). The injection volumes of rutin, isorhamnetin-3-O-rutinoside, vinca saponin, quercetin and kaempferol were 228.60-1 143.00 and 99.60-498.00, 232.20 ~ 1 161.00,22.08 ~ 110.40,15.12 ~ 75.60 ng and the peak area of a good linear relationship, the average recovery was 97.8%, 98.9%, 102.4%, 98.4%, 92.2%. Conclusion The method is simple and accurate and provides the basis for the quality control of flavonoids in the extract of pearl.