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目的探讨模拟难产大鼠盆底肌肉中Dystrophin(抗肌营养不良蛋白)表达的改变和压力性尿失禁发病的关系。方法雌性成年未生产过的Wistar大鼠40只,随机分为四组,D为对照组,其余随机分为A组10只,B组10只,C组10只。A组行阴道扩张术,B组行双侧坐骨神经切除术。C组行阴道扩张+双侧坐骨神经切除术。同样条件饲养1月。取盆底肌肉,采用免疫组织化学方法对Dystrophin表达进行检测。结果实验组与对照组相比盆底肌肉组织中Dystrophin表达减少(P<0.05)。B组、A组、C组中Dystrophin表达逐渐减弱,各组间差异具有显著性(P<0.05)。结论模拟难产大鼠盆底肌肉Dystrophin减少,提示Dystrophin减少可能是SUI发病的因素。
Objective To explore the relationship between the changes of Dystrophin (dystrophin) expression in pelvic floor muscle and the incidence of stress urinary incontinence in simulated dystocia rats. Methods Forty female Wistar rats without adult were randomly divided into four groups. D was the control group. The rest were randomly divided into group A (n = 10), group B (n = 10) and group C (n = 10) Group A was treated by vaginal dilation, while group B was treated by bilateral sciatic nerve resection. C group of vaginal dilation + bilateral sciatic nerve resection. The same conditions feeding January. Pelvic floor muscles were taken and immunohistochemistry was used to detect Dystrophin expression. Results Compared with the control group, the Dystrophin expression in pelvic floor muscle tissue was decreased in the experimental group and the control group (P <0.05). The expression of Dystrophin in group B, group A and group C gradually weakened, with significant difference between groups (P <0.05). Conclusions The reduction of Dystrophin in the pelvic floor muscle of simulated dystocia rats suggests that the decrease of Dystrophin may be the factor of SUI.