论文部分内容阅读
目的了解深圳西乡街道五年来引起感染性腹泻的副溶血弧菌血清型分布、毒力因子及分子流行病学特征,为暴发疫情早期预警预测提供基础数据。方法用传统血清凝集法做血清型鉴定、用荧光PCR法检测毒力基因,再采用脉冲场凝胶电泳(PFGE)对副溶血弧菌进行分子分型。并用BioNumerics软件对PFGE分型图谱进行聚类分析。结果从4 825份标本中,分离出目标致病菌1 077株,其中病原菌副溶血弧菌851株,占79.01%。851株中,563株副溶血弧菌为O3:K6型,占66.15%,其次是O4:K8型,占10.34%。93.4%(795/851)的菌株是tdh+tlh+trh-菌株。对60株O3:K6型副溶血弧菌进行PFGE图谱分析共得到20种不同的PFGE图谱类型,各菌株图谱条带差异小于3条。结论深圳西乡街道五年来副溶血弧菌感染以O3:K6型为主,93.4%的菌株是tdh+tlh+trh-菌株。各菌株图谱具有同源性。
Objective To understand the serotype distribution, virulence factors and molecular epidemiology of Vibrio parahaemolyticus that cause infectious diarrhea in the streets of Xixiang, Shenzhen in the past five years, and provide the basic data for the early warning and prediction of outbreaks. Methods The serotypes were identified by traditional serum agglutination method. The virulence genes were detected by fluorescence PCR and the molecular typing of V. parahaemolyticus was carried out by using pulsed-field gel electrophoresis (PFGE). The PFGE typing patterns were clustered using BioNumerics software. Results A total of 1 077 target pathogens were isolated from 4825 specimens, of which 851 were Vibrio parahaemolyticus, accounting for 79.01%. Of the 851 strains, 563 strains of V. parahaemolyticus were O3: K6 type, accounting for 66.15%, followed by O4: K8 type, accounting for 10.34%. The 93.4% (795/851) strain is the tdh + tlh + trh- strain. A total of 20 different PFGE patterns were obtained from 60 strains of O3: K6 Vibrio parahaemolyticus. The difference of the bands of each strain was less than 3. Conclusion Vibrio parahaemolyticus infection is mainly O3: K6 in the streets of Xixiang, Shenzhen in the past five years, and 93.4% strains are tdh + tlh + trh- strains. Each strain map homology.