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【目的】研究不同激素浓度和组合对红茄不定芽及根诱导的影响,建立红茄的高频再生体系,为开展茄子的遗传转化研究奠定基础。【方法】以红茄子叶为外植体,采用0.5～2.0mg/L6-BA、0.1～0.5mg/L IAA激素组合对不定芽进行诱导,采用0～0.5mg/L IAA对根进行诱导,筛选出最佳的分化培养基和生根培养基。【结果】在不定芽诱导培养基中,当6-BA浓度在0.5～2.0mg/L时,随着浓度增加,不定芽分化率逐渐升高;当IAA浓度在0.1～0.5mg/L时,随着IAA浓度的增加,不定芽萌发率呈先增加后降低趋势,其中以添加6-BA2.0mg/L、IAA0.3mg/L的诱导效果最好,分化率高达86%。在根诱导培养基中,随着IAA浓度的增加,根诱导率呈先降低后升高趋势,诱导率可达100.0%。【结论】红茄子叶不定芽诱导最佳培养基为MS+2.0mg/L6-BA+0.3mg/L IAA;不定芽生根最佳培养基为MS+0.1mg/L IAA。
【Objective】 The effects of different hormone concentrations and combinations on adventitious shoots and root induction of R. solanacearum were studied, and the high-frequency regeneration system of R. solanacearum was established to lay the foundation for the genetic transformation of eggplant. 【Method】 The adventitious buds were induced by using red eggplant leaf explants with 0.5-2.0 mg / L 6-BA and 0.1-0.5 mg / L IAA hormones. The roots were induced with 0-0.5 mg / L IAA, Select the best differentiation medium and rooting medium. 【Result】 The results showed that when the concentration of 6-BA was 0.5-2.0 mg / L, the differentiation rate of adventitious buds gradually increased with the increasing of concentration. When IAA concentration was 0.1-0.5 mg / L, With the increase of IAA concentration, the germination rate of adventitious buds increased firstly and then decreased. The best induction effect was 6 mg / L 6-BA and IAA 0.3 mg / L, the differentiation rate was as high as 86%. In root induction medium, with the IAA concentration increased, the root induction rate first decreased and then increased, the induction rate of up to 100.0%. 【Conclusion】 Adventitious shoots induced by red soja were best cultured in the medium of MS + 2.0mg / L 6-BA + 0.3mg / L IAA. Adventitious buds were best rooted for MS + 0.1mg / L IAA.