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目的研究山茱萸美拉德反应产物(MRPs)对四氯化碳(CCl4)诱导的小鼠肝损伤的保护作用。方法小鼠连续给药7 d后,采用CCl4诱导急性肝损伤,观察小鼠血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的活性变化,测定肝脏指数及肝组织的丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-Px)水平,并采用HE染色对肝组织进行病理学检查,评价山茱萸MRPs对小鼠急性肝损伤的保护作用。结果与模型组比较,各给药组小鼠肝脏指数及血清ALT、AST活性均显著降低(P<0.05,P<0.01);肝组织MDA含量也显著降低(P<0.01),阳性对照组和超滤高剂量组GSH-Px活力水平显著升高(P<0.01,P<0.05)。病理切片显示,MRPs各给药组可以不同程度地改善肝脏组织病变。结论山茱萸MRPs对急性CCl4中毒小鼠受损肝脏有明显的保护作用。
Objective To study the protective effects of Maillard reaction products (MRPs) from Cornel on CCl4-induced liver injury in mice. Methods The mice were administered with CCl4 for 7 days. After acute liver injury induced by CCl4, the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum of mice were observed. The liver index and Liver tissue MDA content and glutathione peroxidase (GSH-Px) levels, and HE staining for pathological examination of liver tissue to assess the protective effect of Cornus officinalis MRPs on acute liver injury in mice . Results Compared with the model group, the liver index and serum ALT and AST activities of the mice in each administration group were significantly decreased (P <0.05, P <0.01), and the MDA content in the liver tissue was also significantly decreased (P <0.01) The GSH-Px activity in the ultrafiltration high-dose group was significantly increased (P <0.01, P <0.05). Pathological sections showed that MRPs in each administration group could improve liver tissue lesion to some extent. Conclusion Cornus officinalis MRPs have a significant protective effect on the damaged liver of acute CCl4-poisoned mice.