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目的探讨松花粉对脑衰老的作用及其相关机制。方法以100mg/kg D-半乳糖皮下注射建立小鼠衰老模型,灌胃给予模型小鼠高、中、低三个剂量(500mg/kg、1000mg/kg、1500mg/kg)的松花粉,并以非酶糖基化抑制剂盐酸氨基胍(AG)为阳性对照,连续给药8周后,采用跳台法测定小鼠的学习记忆能力。处死小鼠获取血清及脑组织,检测晚期糖基化终末产物(AGE)水平,超氧化物歧化酶(SOD)活力、MDA水平以及炎症因子IL-6和TNF-α水平等衰老相关指标。结果跳台法检测模型组较正常组潜伏期显著缩短,5min内错误次数显著增加(P<0.05),而松花粉处理后能显著逆转这一变化(P<0.05),高剂量组效果最佳;同时,松花粉处理组能显著抑制模型组血清及脑组织中AGE水平及IL-6、TNF-α水平的升高(P<0.05),提高血清及脑组织SOD活力(P<0.05)并下调MDA水平(P<0.01)。结论松花粉能显著改善衰老模型小鼠的学习记忆功能,并上调抗氧化活性和降低衰老相关的炎症介质水平,抑制体内非酶糖基化可能是其发挥延缓衰老作用的机制之一。
Objective To investigate the effect of pine pollen on brain aging and its related mechanisms. Methods The mouse model of aging was established by subcutaneous injection of 100mg / kg D-galactose. The model mice were given intragastric administration of three high, middle and low doses of pine pollen (500mg / kg, 1000mg / kg, 1500mg / kg) Aminoguanidine hydrochloride (AG), a nonenzymatic glycosylation inhibitor, was used as a positive control. After 8 consecutive weeks of administration, the learning and memory abilities of mice were determined by the step-down method. The mice were killed to obtain the serum and brain tissue. The levels of advanced glycation end products (AGEs), superoxide dismutase (SOD), MDA, IL-6 and TNF- Results Compared with the normal group, the latency of the model was significantly shortened (P <0.05) and the pollen treatment significantly reversed this change (P <0.05), and the high dose group was the best. At the same time, , Pine pollen treatment group can significantly inhibit the serum and brain tissue AGE levels and IL-6, TNF-α levels (P <0.05), increase serum and brain SOD activity (P <0.05) and down MDA Level (P <0.01). Conclusion Pine pollen can significantly improve the learning and memory function, increase the anti-oxidative activity and reduce the level of inflammatory mediators related to aging, and inhibit the nonenzymatic glycosylation in vivo may be one of the mechanisms that may play an anti-aging role.