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以柠条根际土壤为试材,采用CAS(铬天青S)选择性培养基分离筛选具有产铁载体功能的根际促生菌株,通过16S r DNA序列分析进行菌株鉴定,并测定菌株解磷、固氮、产IAA(吲哚乙酸)和ACC(1-氨基环丙烷-1-羧酸)脱氨酶等促生活性,通过盆栽试验分析验证菌株Fe11对柠条幼苗生长的影响。结果表明:共分离出14株产铁载体根际促生菌,其中6株菌具备较强的产铁载体能力。所有菌株的16S r DNA序列同源性与假单胞菌达到99%~100%,假单胞菌属细菌是柠条根际产铁载体的优势菌群。多数菌株兼具2种以上促生活性,菌株Fe9、Fe11和Fe13表现出多种促生潜能。回接试验证实,接种菌株Fe11使柠条幼苗的株高、根长、叶片数、鲜质量和干质量分别增长17.25%、6.68%、17.75%、42.51%和20.67%,表明菌株Fell对柠条的生长具有良好的促进效果。
Rhizosphere soils of Caragana korshinskii rhizosphere soil were used as materials to isolate rhizosphere-promoting strains with the function of iron-producing vector by using CAS (Chrysanthemum S) selective medium. The strains were identified by 16S r DNA sequence analysis, Phosphorus, nitrogen fixation, IAA (indole acetic acid) and ACC (1-aminocyclopropane-1-carboxylic acid) deaminase and other pro-activity, the pot experiment was conducted to verify the effect of strain Fe11 on the growth of Caragana korshinskii seedlings. The results showed that a total of 14 rhizosphere-promoting bacteria were produced, of which 6 strains had strong iron-producing capacity. The 16S r DNA sequence homology of all isolates reached 99% ~ 100% with that of Pseudomonas. Pseudomonas was the dominant microflora of rhizosphere producing iron carrier. Most strains have more than two kinds of promoting activity, strains Fe9, Fe11 and Fe13 showed a variety of growth potential. The back-connect test confirmed that the inoculation strain Fe11 increased the plant height, root length, leaf number, fresh weight and dry weight of Caragana korshinskii seedlings by 17.25%, 6.68%, 17.75%, 42.51% and 20.67% The growth has a good promotion effect.