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[目的]研究细胞分裂素与大白菜根肿病的关系,探讨大白菜根肿病的发病机理。[方法]从发病植株的肿瘤提取孢子,再提取孢子DNA进行PCR检测。将鉴定出的根肿菌孢子于播种期、发芽期以及发芽后21 d接种到三角瓶内的培养土中,于发芽期在培养土中添加0.08 μmol/L6-BA。从接菌植株长出的肿瘤提取孢子,对孢子进行电镜检验,并统计 6-BA 处理及对照的根肿病发病率。[结果]在三角瓶内,根肿菌可引起大白菜植株发病;添加0.08 μmol/L6-BA处理的根肿病发病率为100%,未加细胞分裂素处理的发病率为57%,前者肿瘤体积也明显大于后者。扫描电镜显示大白菜根肿菌休眠孢子的大小为1.5～4.3 μm。[结论]6-BA能明显促进大白菜根肿病肿瘤的形成。
[Objective] The research aimed to study the relationship between cytokinin and clubroot disease in cabbage and to explore the pathogenesis of cabbage clubroot. [Method] The spores were extracted from the tumor of the diseased plants and then the spore DNA was extracted for PCR detection. The identified root blister spores inoculation, germination and 21 d after germination inoculated into the triangular flask culture soil, in the germination period added 0.08 μmol / L 6-BA. The spores were extracted from the tumors grown in the inoculated plants, and the spores were examined by electron microscopy. The incidence of clubroot disease was also calculated by 6-BA treatment and control. [Result] The root blight bacteria could cause the emergence of cabbage plants in the flask. The incidence of clubroot disease was 100% with addition of 0.08 μmol / L 6-BA, and 57% with no cytokinin treatment Tumor volume was also significantly greater than the latter. Scanning electron microscopy showed that the size of resting spores of P. brassicae was 1.5-4.3 μm. [Conclusion] 6-BA can significantly promote the formation of clubroot cancer in Chinese cabbage.