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通过环糊精的聚合反应及其对碳纳米管的分散作用制备出不溶于水的聚环糊精-碳纳米管复合导电修饰材料,把CNT的稳定性、导电性和催化活性与环糊精分子的包络作用及生物相容性结合起来,得到一种可用于制备电化学生物传感器的碳纳米管-聚合物复合材料。在此基础上,通过优化固定化方法,在该复合材料上固定乙酰胆碱酯酶,制备出了灵敏度较高、线性范围较宽的有机磷农药生物传感器,该传感器可以在1.0~15.0 mg/L浓度范围内检测农药甲胺磷的含量,检测下限为0.05 mg/L。利用不同扫描速度下的循环伏安数据分析了传感器的电极反应机理,发现该传感器的电极过程由扩散步骤和电子转移步骤混合控制。
Through the polymerization of cyclodextrin and its dispersion of carbon nanotubes, a water-insoluble polycyclodextrin-carbon nanotube composite conductive modification material was prepared, and the stability, conductivity and catalytic activity of the CNTs were mixed with the cyclodextrin Molecular envelopment and biocompatibility combined to obtain an electrochemical biosensor can be used for the preparation of carbon nanotube - polymer composite material. On this basis, by optimizing the immobilization method, the acetylcholinesterase was immobilized on the composite material to prepare the organophosphorus pesticide biosensor with high sensitivity and wide linear range. The sensor can be used in the concentration range of 1.0-15.0 mg / L Detection of pesticide methamidophos within the range, the detection limit of 0.05 mg / L. The electrode reaction mechanism of the sensor was analyzed by using the cyclic voltammetry data at different scan rates. The electrode process of the sensor was found to be a mixture of diffusion and electron transfer steps.