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目的:对手足口病患者标本进行病毒核酸检测,探讨实时荧光定量RT-PCR方法在手足口病检测中的意义。方法:用实时荧光定量RT-PCR方法对2011年度淮北市采集的257份标本进行肠道病毒核酸检测,并对肠道病毒(EV71)、柯萨奇病毒A组16型(CoxA16)进行分型;同时,与常规RT-PCR的方法进行了比对。结果:257份标本中肠道病毒核酸阳性138份,阳性率为53.70%,其中,EV71阳性标本89份,阳性率34.63%,CoxA16型核酸阳性34份,阳性率13.23,未分型15份,占病例总数的5.84%。结论:实时荧光定量RT-PCR方法耗时短、特异性强、灵敏度高,可作为手足口病检测的诊断方法。
Objective: To detect viral nucleic acid in hand, foot and mouth disease patients and explore the significance of real-time fluorescence quantitative RT-PCR in the detection of hand-foot-mouth disease. Methods: 257 specimens collected from Huaibei in 2011 were tested for enterovirus nucleic acid by real-time fluorescent quantitative RT-PCR. The enterovirus (EV71) and CoxA16 were genotyped ; At the same time, compared with the conventional RT-PCR method. Results: Of the 257 specimens, 138 were positive for enterovirus nucleic acid with a positive rate of 53.70%. Among them, 89 were positive for EV71, 34.63% for positive samples, 34 were positive for CoxA16, 13.23 for positive samples, 15 were not classified, Accounting for 5.84% of the total number of cases. Conclusion: Real-time fluorescence quantitative RT-PCR method is short, time-consuming, specific and sensitive and can be used as a diagnostic method for detection of HFMD.