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目的:研究L-精氨酸枕大池注射对兔蛛网膜下腔出血后血管痉挛的影响及作用机制。方法:采用双侧颈动脉结扎及枕大池二次注血法制造兔蛛网膜下腔出血模型。在蛛网膜下腔出血后第4天,以比色法测定血清及脑脊液中一氧化氮及脑组织的浓度。光镜下测定基底动脉的动脉壁厚度和基底动脉的内径,以其比值作为脑血管痉挛的指标。治疗组分为300μmol/L及500μmol/L组,在蛛网膜下腔出血后第4天,枕大池持续微泵滴注L-精氨酸,在滴注后再分别研究上述指标。结果:蛛网膜下腔出血后第4天,基底动脉的动脉壁厚度和内径的比值(0.085±0.007)明显升高(t=20.26,P<0.05);血清一氧化氮犤(24.34±7.36)μmol/L,t=6.72,P<0.05犦及脑脊液中一氧化氮犤(10.68±3.43)μmol/L,t=4.25,P<0.05犦及脑组织NOS犤(0.007±0.001)nmol/(s·g),t=6.14,P<0.05犦.的浓度降低。在L-精氨酸滴注后,脑血管痉挛缓解。血清一氧化氮犤(37.38±6.42)μmol/L,t=3.27,P<0.05犦、脑脊液中一氧化氮犤(14.16±3.36)μmol/L,t=1.98,P<0.05犦脑组织一氧化氮合酶(nitricoxygenase,NOS)犤(0.011±0.002)nmol/(s·g),t=3.10,P<0.05犦的浓度较对照组明显升高。结论:L-精氨酸枕大池注射对兔蛛网膜下腔出血后的血管痉挛具有治疗作用。L-精氨酸可能通过影响一氧化氮及NOS而
Objective: To study the effect and mechanism of L-arginine injection into the cistern of spleen after subarachnoid hemorrhage in rabbits. Methods: Rabbit models of subarachnoid hemorrhage were made by bilateral carotid artery ligation and occipital cistern injection. On the fourth day after subarachnoid hemorrhage, the concentrations of nitric oxide and brain tissue in serum and cerebrospinal fluid were measured by colorimetry. The thickness of the artery wall and the diameter of the basilar artery in the basilar artery were measured under a light microscope, and the ratio thereof was used as an index of cerebral vasospasm. The treatment group consisted of 300μmol / L and 500μmol / L groups. On the 4th day after subarachnoid hemorrhage, L-arginine was continuously injected into the cisterna magna by micro-pump, and the above indexes were studied after instillation. Results: On the 4th day after subarachnoid hemorrhage, the ratio of arterial wall thickness and internal diameter (0.085 ± 0.007) in basilar artery was significantly higher (t = 20.26, P <0.05), serum nitric oxide 犤 (24.34 ± 7.36) (10.68 ± 3.43) μmol / L, t = 4.25, P <0.05 犦 and brain tissue NOS 犤 (0.007 ± 0.001) nmol / (s · G), t = 6.14, P <0.05 犦. After L-arginine instillation, cerebral vasospasm was relieved. The level of serum nitric oxide (.3 (37.38 ± 6.42) μmol / L, t = 3.27, P <0.05 犦) and cerebrospinal fluid nitric oxide 犤 (14.16 ± 3.36) μmol / L, t = 1.98, Nitric oxide synthase (NOS) 犤 (0.011 ± 0.002) nmol / (s · g), t = 3.10, P <0.05 犦 concentration was significantly higher than the control group. Conclusion: The injection of L-arginine occipital cistern has a therapeutic effect on vasospasm after subarachnoid hemorrhage in rabbits. L-arginine may affect nitric oxide and NOS