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小鼠每天腹腔注射４－氨公－２’，４’－二氯二苯醚（２’，４’－ｄｉｃｌ）及４－氨基－４’－甲基二苯醚（４’－ＣＨ３）４天，于末次给药２４ｈ后实验，发现：（１）以小鼠戊巴比妥睡眠时间作为体内Ｐ４５０活性指标，４’－ＣＨ３组Ｐ４５０活性高于生理盐水组，而２’，４’－ｄｉＣｌ组小鼠体内Ｐ４５０活性低于生理盐水组。（２）两处理组小鼠体外测得肝微粒体Ｐ４５０含量均显著高于生理盐水组（Ｐ＜０．０５），低于苯巴比妥组。（３）２’，４’－ｄｉＣｌ组小鼠由ｚｏｘａｚｏｌａｍｉｎｅ引起的瘫痪时间显著高于生理盐水组（Ｐ＜０．０１），提示该化合物对体内Ｐ４４８活性亦有抑制作用。体外实验也发现，两者对ＢＮＦ诱导的大鼠肝微粒体Ｐ４４８的活性均有不同程度的抑制。
Mice were injected intraperitoneally with 4-amino-2 ’, 4’-diclodiphenyl and 4-amino-4’-methyldiphenylether Day, after the last administration for 24 h, we found: (1) The pentobarbital sleep time in mice was used as an indicator of P450 activity in vivo. The P450 activity in 4’-CH3 group was higher than that in saline group, while the 2 ’, 4’- The P450 activity of diCl group mice was lower than that of saline group. (2) The content of P450 in liver microsomes in both treatment groups was significantly higher than that in saline group (P <0.05) and lower than phenobarbital group. (3) The time of paralysis caused by zoxazolamine in 2 ’, 4’-diCl group mice was significantly higher than that in saline group (P <0.01), suggesting that the compound also inhibited P448 activity in vivo. In vitro experiments also found that both of the BNF-induced rat liver microsomal P448 activity were inhibited to varying degrees.