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目的 探讨脑内一氧化氮在大鼠痛觉调制中的作用。方法 以钾离子透入引起大鼠甩尾反应的电流强度作为痛反应指标 ,观察侧脑室注射NG-硝基 -L -精氨酸甲酯、NG 硝基 D 精氨酸甲酯、硝普钠和血红蛋白等 ,对大鼠痛阈的影响。结果 侧脑室注射一氧化氮合酶抑制剂NG-硝基 -L -精氨酸甲酯后 ,大鼠痛阈明显增加 ;而注射NG-硝基 -D -精氨酸甲酯则对痛阈无明显影响。侧脑室注射一氧化氮供体物质硝普钠后 ,痛阈明显降低 ;而注射硝普钠与血红蛋白的混合液和Hb则对痛阈无显著影响。结论 大鼠脑内NO水平降低有镇痛作用 ,而NO水平的升高则有痛敏作用。提示 ,大鼠脑内NO在痛觉调制的复杂过程中 ,发挥着重要作用。
Objective To investigate the role of nitric oxide in rat pain modulation. Methods The current intensity induced by potassium ion penetration in rat tail flick reaction was taken as the index of pain reaction. The intracerebroventricular injection of NG-nitro-L-arginine methyl ester, NG nitro-D arginine methyl ester and sodium nitroprusside And hemoglobin, on the pain threshold in rats. Results Intracerebroventricular injection of nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester significantly increased the pain threshold in rats, while injection of NG-nitro-D-arginine methyl ester on pain threshold No significant effect. Intracerebroventricular injection of nitric oxide donor sodium nitroprusside pain threshold decreased significantly; and sodium nitroprusside and hemoglobin and Hb injected on the pain threshold had no significant effect. Conclusion The decrease of NO level in rat brain has analgesic effect, while the increase of NO level has the effect of pain-sensitization. It is suggested that NO in rat brain plays an important role in the complex process of pain modulation.