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以起源于组织培养的一个苹果品种和一个砧木(Elstar和M9)的幼龄盆栽植株作试材,在其茎节以下进行表皮刻伤,然后将发根土壤杆菌(Agrobacterium rhizogenes)的LBA 8490或LBA9365菌系接种于伤口,并用石棉包裹,外缠塑料薄膜。大约9周后,接种部位均产生了大量根,将这些新生根埋在填满土的圆筒中,可使其迅速生长。2周后,将已具有新生根的新梢从母株上剪下,进行盆栽。对该种繁殖方法(特别是砧木)的优点和可行性进行了讨论。(文摘号3598) 译自(英) 《园艺学文摘》,1989年,59卷,5期译者:董启凤
A young potted plant derived from one apple cultivar and one rootstock (Elstar and M9) from tissue culture was used as a test material, epidermal wounding was performed below its stem, and then Agrobacterium rhizogenes LBA 8490 or LBA9365 strain inoculated on the wound, and wrapped with asbestos, wrapped around the plastic film. Approximately 9 weeks later, a large number of roots were produced at the inoculation site, and the new roots were buried in a soil-filled cylinder to allow rapid growth. Two weeks later, new shoots with new roots were cut from the mother plants and potted. The advantages and feasibility of this breeding method, especially rootstock, are discussed. (Abstracts No. 3598) translated from (British) “Gardening Abstracts”, 1989, Volume 59, Issue 5 Translator: Dong Qi Feng