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肾综合征出血热(HFRS)是全国重点防治的自然疫源性疾病,根据卫生部关于HFRS防治监测方案要求,结合我市HFRS防治工作安排,1996~1998年连续对人间疫情、鼠间疫情进行了监测,现将结果报告如下。材料与方法1．人间疫情资料:来源于本站疫情统计室,人口资料来源于市统计局。2．鼠情监测资料:以夹夜法捕鼠计算鼠密度,捕到鼠后进行编号登记,无菌操作取肺,-85℃冰箱冻存待检HFRS抗原。3．诊断符合率:采集临床确诊HFRS病人急性期和恢复期血清,低温冰箱保存待检。4．实验方法:以间接免疫荧光法(TFAT),日本产Olympus落射光荧光显微镜下检测HFRS抗原、抗体。
Hemorrhagic fever with renal syndrome (HFRS) is a natural foci of key national prevention and treatment. According to the requirements of the Ministry of Health on HFRS prevention and control programs and the HFRS prevention and control work schedule in our city, from 1996 to 1998, Monitoring, the results are reported below. Materials and methods 1. Epidemic situation information: from the station epidemic situation statistics, population data from the Municipal Bureau of Statistics. 2. Rodent surveillance data: The rat density was calculated by catching the night with the trap method, and the number of the rat was registered after catching the mouse. The aseptic technique was used to take the lung and the HFRS antigen was frozen in the refrigerator at -85 ℃. 3. Diagnostic coincidence rate: collection of clinically diagnosed HFRS patients with acute and convalescent serum, cryogenic refrigerator to be seized. 4. Experimental methods: HFRS antigen and antibody were detected by indirect immunofluorescence assay (TFAT) and Olympus epi-light fluorescence microscope in Japan.