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作者用脊髓灰质炎病毒Ⅰ型代表肠道病毒,将市售的19种饮料和液汁进行抗病毒效果试验。各种饮料和液汁均以其自然状态的 pH 和用1 N 氢氧化钠溶液校正其 pH 至7.0时,各取2 ml 置螺口瓶中,并用2 ml 水作对照。在各瓶内加入0.05 ml 约形成8,000个空斑单位的上述病毒的悬液,置22℃培养2小时后,用含10%胎牛血清的199培养液作成1:100稀释,然后各三次取2.5 ml 加入
The authors used poliovirus type I to represent enteroviruses and tested 19 commercially available beverages and juices for antiviral efficacy. Various beverages and juices were taken in 2 ml home-screw bottles at their natural pH and pH adjusted to 7.0 with 1 N sodium hydroxide solution and control with 2 ml of water. To each bottle was added 0.05 ml of a suspension of the above virus forming about 8,000 plaque units and incubated at 22 ° C for 2 hours. The suspension was diluted 1: 100 with 199 medium containing 10% fetal bovine serum and then each of three 2.5 ml added