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背景:研究表明,移植后的骨髓间充质干细胞在新的环境中能够被诱导分化为神经元样细胞,从而替代损伤细胞重建神经环路。目的:建立大鼠骨髓间充质干细胞和神经细胞的体外共培养系统,观察共培养条件下神经细胞对骨髓间充质干细胞向神经元样细胞分化的影响。方法:体外培养Wistar大鼠脑组织神经细胞和股骨骨髓间充质干细胞,采用Transwell小室共培养分化诱导,观察骨髓间充质干细胞的组织形态变化,在共培养第5天免疫荧光染色检测骨髓间充质干细胞中神经细胞的特异标志物;与对照组单纯骨髓间充质干细胞培养结果相比较。结果与结论:神经细胞共培养系统中的骨髓间充质干细胞生长伸展,呈放射状,互相形成连接,特异性烯醇化酶显示阳性结果,具有神经元样细胞的特性,表达特异性烯醇化酶阳性的神经元比例可达(33.0±10.5)%。而对照组骨髓间充质干细胞未形成神经元样细胞的形态结构,免疫荧光显示特异性烯醇化酶阴性。提示神经细胞提供的微环境对骨髓间充质干细胞分化为神经元样细胞具有诱导分化作用。
BACKGROUND: Studies have shown that, after transplantation, bone marrow mesenchymal stem cells can be induced to differentiate into neuron-like cells in a new environment, thereby replacing the damaged cells in rebuilding the neural circuit. OBJECTIVE: To establish an in vitro co-culture system of rat bone marrow mesenchymal stem cells and neural cells and observe the influence of neural cells on the differentiation of bone marrow mesenchymal stem cells into neuron-like cells under co-culture conditions. Methods: Neural cells and bone marrow mesenchymal stem cells of Wistar rats were cultured in vitro. Transwell chamber co-culture was used to induce differentiation. Morphological changes of bone marrow mesenchymal stem cells were observed. Immunofluorescence staining was used to detect bone marrow Specific markers of neurons in MSCs were compared with those of MSCs cultured in control group. RESULTS AND CONCLUSION: Bone marrow-derived mesenchymal stem cells in nerve cell co-culture system grew in a radial pattern and connected with each other. Specific enolase showed positive results, which showed the characteristics of neuron-like cells. The expression of specific enolase-positive Of the neurons up to (33.0 ± 10.5)%. While the control group did not form the morphological structure of neuron-like cells, immunofluorescence showed specific enolase negative. It is suggested that the microenvironment provided by nerve cells can induce the differentiation of BMSCs into neuron-like cells.