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目的探讨小儿肺炎支原体感染合并支气管哮喘患儿血浆中的肿瘤坏死因子-α(TNF-α)与人内皮素-1(ET-1)检测的临床意义。方法选取该院2011年1月-2013年7月间收治的支原体肺炎合并支气管哮喘患儿41例,采用酶免疫吸附法(ELISA)对41例患儿相关的血浆细胞因子ET-1水平、TNF-α水平进行测定,并与30例正常健康儿童对照组比较。观察组中患儿经治疗后症状体征缓解,对其进行8个月的跟踪随访,记录哮喘急性发作的次数以及严重程度。结果 41例肺炎支原体感染患儿在急性发作期以及缓解期的血浆ET-1、TNF-α水平均明显高于正常人群,且与缓解期比较急性发作期的ET-1、TNF-α水平明显升高,以上数据比较差异均有统计学意义(P<0.05)。血浆ET-1水平越高支气管哮喘患儿在进行8个月内的随访中其哮喘的急性发作次数越多,且呈正相关关系(r=0.501,P<0.01)。血浆TNF-α水平越高,支气管哮喘患儿病情的发作程度更严重(r=0.492,P<0.01),8个月内的平均发作程度越重(r=0.449,P<0.01),统计均呈正相关关系。结论支气管哮喘患儿在急性发作期其血浆ET-1以及TNF-α水平升高,与疾病的预后有关:血浆ET-1水平越高,急性发作的次数就越多;血浆TNF-α水平越高,则发作的程度越严重。
Objective To investigate the clinical significance of detecting plasma levels of tumor necrosis factor-α (TNF-α) and human endothelin-1 (ET-1) in children with mycoplasma pneumoniae infection complicated with bronchial asthma. Methods Forty-one patients with mycoplasma pneumonia and bronchial asthma admitted from January 2011 to July 2013 in our hospital were enrolled in this study. The levels of ET-1, TNF-α and TNF-α in 41 children were determined by enzyme-linked immunosorbent assay (ELISA) -α levels were measured and compared with 30 normal healthy children control group. In the observation group, the symptom and symptom were relieved after treatment. The patients were followed up for 8 months and the number and severity of acute exacerbation of asthma were recorded. Results The levels of plasma ET-1 and TNF-α in 41 cases of Mycoplasma pneumoniae infection in acute attack and remission stages were significantly higher than those in normal population, and the levels of ET-1 and TNF-α in acute attack stage were significantly higher than those in remission stage Increased, the above data were statistically significant differences (P <0.05). The higher the level of plasma ET-1, the more children with bronchial asthma in the follow-up within 8 months, the more the number of acute exacerbation of asthma, and a positive correlation (r = 0.501, P <0.01). The higher the level of plasma TNF-α, the more severe the onset of asthma in children with bronchial asthma (r = 0.492, P <0.01), the heavier the average degree of attack within 8 months (r = 0.449, P <0.01) There was a positive correlation. Conclusion The levels of plasma ET-1 and TNF-α in children with bronchial asthma during acute exacerbation are related to the prognosis of the disease. The higher the level of plasma ET-1, the more the number of acute exacerbations. The higher the level of plasma TNF-α High, the more serious the extent of the attack.