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目的研究长白山地区钝叶瓦松Orostachys malacophyllus茎中活性成分。方法使用色谱法分离钝叶瓦松甲醇提取物的醋酸乙酯萃取物中的化合物,利用核磁共振波谱、质谱等技术进行结构鉴定,采用CCK-8方法测试活性。结果从钝叶瓦松茎甲醇提取物中分离鉴定出5个成分,分别为苹果酸-1-乙酯(1)、2-(3,4-二羟基苯酰基氧代)-苹果酸单乙酯(2)、2-(3,4-二羟基苯酰基氧代)-苹果酸二甲酯(3)、2-对羟基苯酰基氧代-苹果酸二甲酯(4)、邻苯二甲酸二异丁酯(5)。结论化合物1~5为从该属植物中首次分离得到,其中化合物2是新化合物,命名为瓦松酚酸;其对He La细胞的细胞毒活性的IC_(50)值为111.5μmol/L。
Objective To study the active constituents from the stem of Orostachys malacophyllus in Changbai Mountain. Methods Chromatography was used to separate the compounds from the ethyl acetate extract of Pinus Elliottii extract. The structures of the compounds were identified by nuclear magnetic resonance spectroscopy and mass spectrometry. The activity of the compounds was tested by CCK-8 method. Results Five components were isolated and identified from methanolic extracts of Brassica campestris, which were 1-ethyl-1-malate (1), 2- (3,4-dihydroxybenzoyloxy) (2), dimethyl 2- (3,4-dihydroxybenzoyloxy) -malate (3), dimethyl 2-p-hydroxybenzoyloxy-malate (4), o- Diisobutyl formate (5). CONCLUSION Compounds 1 to 5 were isolated from this genus for the first time. Among them, compound 2 is a new compound named as guavane acid. Its IC50 value on HeLa cell cytotoxicity was 111.5 μmol / L.