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目的 通过对川崎病(KD)患儿血清基质金属蛋白酶(MMP)-9及其抑制物组织基质金属蛋白酶抑制物(TIMP)-1水平的测定,探讨MMP-9及TIMP-1与川崎病发病及其冠状动脉并发症发生发展的关系。方法 采用酶联免疫吸附检测(ELISA)法对30例KD无冠状动脉病变(CAL)及9例KD合并CAL患儿急性期和恢复期血清MMP-9、TIMP-1水平进行检测,并与15例其他发热性疾病患儿及18例正常健康儿童进行对照。结果急性期KD有CAL组和无CAL组血清MMP-9、TIMP-1、MMP-9／TIMP-1分别是896.2±81.7、342.6±43.1、2.5±0.6和284.3±40.9、389.5±20.8、0.8±0.2,均较发热对照组及正常对照组(87.9±18.9、251.5±13.0、0.3±0.1和24.6±2.8、90.0±4.2、0.3±0.02)明显增加(P<0.01),且有CAL组的MMP-9及MMP-9／TIMP-1较无CAL组增高更为显著(P<0.01);恢复期KD无CAL组患儿血清MMP-9、TIMP-1、MMP-9／TIMP-1降至正常水平(26.4±7.6、95.6±5.8、0.2±0.1),而有CAL组患儿虽较急性期亦明显下降(220.0±28.3、258.9±15.0、0.9±0.3),但仍然明显高于正常对照组(分别P<0.01和P<0.05),发热对照组血清MMP-9及TIMP-1水平亦较正常对照组明显增高(P<0.01),但其MMP-9／TIMP-1与正常对照组比较差异无显著性(P>0.05)。结论 MMP-9及TIMP-1参与了川崎病的病理生理过?
Objective To investigate the relationship between MMP-9, TIMP-1 and Kawasaki disease in serum of patients with Kawasaki disease (KD) by measuring the levels of serum matrix metalloproteinase (MMP-9) and tissue inhibitor of matrix metalloproteinase-1 And its coronary complications related to the development. Methods Serum levels of MMP-9 and TIMP-1 in 30 patients with KD without coronary artery disease (CAL) and 9 with KD with CAL were detected by enzyme-linked immunosorbent assay (ELISA) Cases of other children with febrile disease and 18 normal healthy children were compared. Results Serum levels of MMP-9, TIMP-1, MMP-9 / TIMP-1 in KD with and without CAL were 896.2 ± 81.7, 342.6 ± 43.1, 2.5 ± 0.6 and 284.3 ± 40.9, 389.5 ± 20.8 and 0.8 ± 0.2, which were significantly higher than those in fever control group and normal control group (87.9 ± 18.9,251.5 ± 13.0,0.3 ± 0.1 and 24.6 ± 2.8,90.0 ± 4.2,0.3 ± 0.02) (P <0.01) MMP-9 and MMP-9 / TIMP-1 were significantly higher than those without CAL (P <0.01). The serum levels of MMP-9, TIMP-1 and MMP-9 / TIMP- (26.4 ± 7.6,95.6 ± 5.8,0.2 ± 0.1), while those in the CAL group were significantly lower than those in the acute phase (220.0 ± 28.3,258.9 ± 15.0 and 0.9 ± 0.3), but still significantly higher than the normal The levels of MMP-9 and TIMP-1 in the fever control group were also significantly higher than those in the control group (P <0.01 and P <0.05, respectively), but the levels of MMP-9 / TIMP-1 in the fever control group were significantly higher than those in the control group There was no significant difference between the two groups (P> 0.05). Conclusion MMP-9 and TIMP-1 are involved in the pathophysiology of Kawasaki disease.