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利用原位分子杂交和免疫组化方法对１４２例乙型肝炎活检肝组织进行丁型肝炎病毒ＲＮＡ及其抗原的定位研究。２８／１４２例丁型肝炎病毒标记阳性。其中慢性重症型６例；慢性活动性１７例；慢性持续性５例。慢性活动性乙肝重叠丁型肝炎病毒感染组发生早期肝硬变的比例明显高于无重叠感染组（Ｐ＜０．０５）。２８例丁型肝炎病毒感染肝组织１９例ＨＢｃＡｇ阳性，并以核浆型为主，提示活动性ＨＢＶ复制与ＨＤＶ感染的正相关性，两者相加作用导致肝损害加重并加速发展为肝纤维化。ＨＤＶＲＮＡ在肝细胞内大量蓄积，ＨＤＡｇ在碎屑状坏死边缘肝细胞或气球样变肝细胞内呈浆膜型分布，提示ＨＤＶ直接细胞毒在丁型肝炎发病学中的作用。
In situ Hybridization and Immunohistochemistry for the Localization of Hepatitis B Virus RNA and Its Antigen in 142 Hepatitis B Biopsies. 28/142 cases of hepatitis D virus positive mark. Among them, 6 cases were chronic severe type, 17 cases were chronic active and 5 cases were chronic persistent. The proportion of chronic cirrhosis with chronic active hepatitis B overlap hepatitis D virus infection was significantly higher than that without overlapping infection (P <0.05). Of the 28 hepatitis B virus-infected liver tissues, 19 were positive for HBcAg, and mainly nuclear plasmids, suggesting a positive correlation between active HBV replication and HDV infection. The additive effect of these two causes an aggravation of liver damage and accelerated development of hepatic fibrosis The HDVRNA accumulates largely in hepatocytes, and the distribution of HDAg in seronegative hepatocytes or balloon-like hepatocytes is marked by serosal distribution, indicating the role of HDV direct cytotoxicity in the pathogenesis of hepatitis C.