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目的通过检测４种常用细胞因子对白血病细胞株Ｋ５６２／Ｓ及其多药耐药细胞株Ｋ５６２／Ａ０２积蓄柔红霉素（ＤＮＲ）能力的影响，评价细胞因子在抗白血病细胞多药耐药性方面的应用前景。方法用流式细胞仪及荧光法检测重组人α－干扰素（ＩＦＮ－α）、白细胞介素２（ＩＬ－２）、肿瘤坏死因子（ＴＮＦ）、重组人粒－单集落刺激因子（ＧＭ－ＣＳＦ）对Ｋ５６２／Ｓ及Ｋ５６２／Ａ０２积蓄柔红霉素能力的影响。结果ＩＬ－２、ＴＮＦ、ＧＭ－ＣＳＦ作用后Ｋ５６２／Ｓ及Ｋ５６２／Ａ０２细胞株对柔红霉素的积蓄无改变，而ＩＦＮ－α可显著提高耐药细胞系Ｋ５６２／Ａ０２细胞内ＤＮＲ浓度，在１５０ｍｉｎ时升高了４.０１倍（Ｐ＜０.０５）。结论小剂量（５００Ｕ／ｍｌ） α－干扰素作用后，多药耐药细胞株 Ｋ５６２／Ａ０２对抗肿瘤药物ＤＮＲ的积蓄作用大大提高，提示ＩＦＮ－α具有提高耐药细胞株细胞内药物浓度、恢复其药物敏感性、逆转多药耐药性的作用。
Objective To evaluate the effect of four cytokines on the ability of daunomycin (DNR) to accumulate in leukemia cell line K562 / S and its multidrug-resistant cell line K562 / A02, and to evaluate the effect of cytokines on multidrug resistance in leukemia cells The application prospects. Methods The expressions of IFN-α, IL-2, TNF and GM-CSF were detected by flow cytometry and fluorometry. CSF) on the capacity of K562 / S and K562 / A02 to accumulate daunorubicin. Results No significant changes were observed in daunorubicin accumulation in K562 / S and K562 / A02 cells after treatment with IL-2, TNF and GM-CSF, but IFN-α significantly increased DNR concentration in K562 / A02 cell line, 4.01-fold (P <0.05) at 150 min. Conclusions The effect of multidrug-resistant cell line K562 / A02 on the accumulation of antitumor drug DNR greatly increased after the effect of low dose (500U / ml) interferon-α, suggesting that IFN-α could increase the intracellular drug concentration of drug- Its drug sensitivity reverses the role of multidrug resistance.