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本研究的目的是在大鼠神经外膜下注射强地松龙、VB1、VB12和氟美松后，观察是否能引起神经的组织病理学改变。分别将下列药物（2．5％强地松龙；0．5％强地松龙；5％VB1；1％VB1；0．25％VB12；0．05％VB12；0．5％强地松龙＋1．6％利多卡因；0．1％氟美松＋1．6％利多卡因；生理盐水1ml），在手术显微镜下，用皮试针头将药液注入到坐骨神经的神经外膜下，3天至14天后分别取神经标本进行形态学检查，结果显示注射强地松龙、VB1和VB12后，光镜HE染色切片、Teased纤维（单根神经纤维）切片和电镜检查，均可见到髓鞘异常；高浓度（5％）VB1可导致轴突坏死，高浓度（25％）强地松龙，2周和4周后全部均可见到神经内残留有药物结晶。注射生理盐水、VB12和氟美松的动物，仅于3天时可见到炎症，未见神经结构损害。本研究结果提示，临床常用浓度的强地松龙和VB1神经内注射后可引起髓鞘损伤和轴突病变，其病变高峰期持续2周以上。相比之下，氟美松和VB12则安全性较大。
The aim of this study was to observe whether histopathological changes of nerve could be induced after injecting prednisolone, VB1, VB12 and dexamethasone in rat epineurium. The following drugs (2.5% prednisolone; 0.5% prednisolone; 5% VB1; 1% VB1; 0.25% VB12; 0.05% VB12; Dragon + 1.6% lidocaine; 0.1% dexmedetomidine + 1.6% lidocaine; saline 1ml) under a surgical microscope with a skin test needle injected into the sciatic nerve under the epineurium, Three days to 14 days after the nerve specimens were taken for morphological examination showed that injection of prednisolone, VB1 and VB12, light microscope HE stained sections, Teased fiber (single nerve fiber) sections and electron microscopy, can be seen in the pulp Sheath abnormalities were found in high-concentration (5%) VB1. Axon necrosis was induced in high concentration (25%) of prednisolone, and the drug remained in the nerve after 2 and 4 weeks. Injections of normal saline, VB12 and dexamethasone animals showed inflammation only in 3 days and no neuro-structural damage. The results of this study suggest that prednisolone and VB1 injected at clinically useful concentrations may cause myelin damage and axonal lesions with peak lesions lasting more than two weeks. In contrast, flumethasone and VB12 are more secure.