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目的探讨慢性应激对大鼠海马CA3区锥体细胞结构和血清皮质酮浓度的影响。方法将20只雄性Sprague-Dawley大鼠按体质量随机分为应激组和对照组,每组10只。采用高尔基染色法及酶联免疫分析方法,观察慢性强迫游泳应激对大鼠海马CA3区锥体细胞顶树突和血清皮质酮浓度的影响。结果应激组大鼠海马CA3区锥体细胞顶树突的总长度[(112±10)μm]短于对照组[(168±34)μm],差异有统计学意义(P<0.01);一级树突直径[(9.0±1.1)μm]大于对照组[(5.7±0.9)μm],差异有统计学意义(P<0.01);血清皮质酮浓度[(13±14)μg/L]低于对照组[(30±16)μg/L],差异有统计学意义(P<0.05)。结论慢性强迫游泳可引起大鼠海马CA3区锥体细胞顶树突及血清皮质酮浓度的改变。
Objective To investigate the effects of chronic stress on pyramidal cell structure and serum corticosterone concentration in hippocampal CA3 area of rats. Methods Twenty male Sprague-Dawley rats were randomly divided into stress group and control group, with 10 rats in each group. The effects of chronic forced swimming stress on the density of apical dendrites and serum corticosterone in hippocampal CA3 hippocampus of rats were observed by Golgi staining and enzyme-linked immunosorbent assay. Results The total length of apical dendrites in pyramidal cells in hippocampal CA3 region of hippocampus was (112 ± 10) μm in stress group, which was significantly shorter than that in control group [(168 ± 34) μm] (P <0.01). The diameter of primary dendrites [(9.0 ± 1.1) μm] was significantly larger than that of the control group [(5.7 ± 0.9) μm] (P <0.01), and the serum corticosterone concentration was (13 ± 14) μg / L Lower than that of the control group [(30 ± 16) μg / L], the difference was statistically significant (P <0.05). Conclusion Chronic forced swimming can cause changes of apical dendrites and serum corticosterone in rat hippocampal CA3 pyramidal cells.