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目的研究旋毛虫成囊前期幼虫抗原(pre-encystedlarvaantigens,PELA)的组分和诊断价值。方法应用免疫印迹对比分析PELA和成囊幼虫抗原(ELA)的组分和它们对人及大民旋毛虫病诊断的敏感性和特异性。结果PELA有3条抗原带(分子量分别为:15、17和129kD)与ELA明显不同;对于感染旋毛虫的大鼠,PELA的首次血清阳性反应出现在感染后第10天,而ELA则在感染后第14天,大鼠抗PELA血清抗体出现较早(P<0.01);对22份旋毛虫病人血清,PELA的阳性率为100%,ELA为72.7%,差异具有显著性(P<0.05);除1例鞭虫病人外,其它寄生虫病入及健康人血清,两种抗原均呈阴性反应,两种抗原的特异性无显著性差异(P>0.05)。结论PELA比ELA对早期旋毛虫病的诊断著有更大的价值。
Objective To study the components and diagnostic value of pre-encystedlarva antigens (PELA) in Trichinella spiralis. METHODS: The components of PELA and lacunar antigen (ELA) were compared by immunoblotting and their sensitivity and specificity for the diagnosis of human and adult Trichinella were examined. RESULTS: Three bands of PELA (molecular weight 15, 17 and 129 kD, respectively) were significantly different from those of ELA. The first seropositive PELA seroprevalence was observed on day 10 post infection in rats infected with Trichinella, while ELA On the 14th day, the anti-PELA serum antibody appeared earlier (P <0.01). The positive rate of PELA in serum of 22 patients with Trichinella was 100% and ELA was 72.7%, the difference was significant P <0.05). Except for one case of whipworm, all the other parasitic diseases and healthy human serum showed negative response to both antigens, and the specificity of the two antigens was not significantly different (P> 0.05 ). Conclusions PELA is more valuable than ELA in the diagnosis of early stage trichinosis.