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研究分析了土壤紫青霉菌分泌毒素对连作大豆全生育过程的危害．结果表明，在大豆种子萌动期毒素致毒作用已经开始；在大豆幼苗期及分枝期，较高浓度毒素（×１０３倍稀释）使大豆完全不结瘤，极低浓度（×１０５倍稀释）仍然抑制４０％结瘤，致使重茬大豆光合固氮能力显著降低．而大豆残根、凋落物等通过刺激紫青霉菌的增长，造成对大豆进一步的危害．对连作大豆土壤紫青霉毒素的调控研究表明，使用土壤放线菌ＭＢ生物防治，可使重茬大豆增产８．４～１８．９％；使用海洋放线菌ＭＢ９７生物防治可使重茬大豆增产３０．５％．应用ＭＢ９７制剂对连作大豆全生育过程的防病、减毒控制的综合调控，可使重茬５年大豆田间微区试验折合大豆产量达到４５７５ｋｇ·ｈｍ－２．
In this study, we analyzed the harm of the secretion of toxins from Penicillium solani to the whole growth process of continuous cropping soybean. The results showed that toxic effects of toxins had already started during the germinating stage of soybean seeds. At the soybean seedling stage and the branching stage, higher concentrations of toxins (× 103-fold dilution) made soybean completely non-nodulated and very low concentrations (× 105 dilution) Still inhibit 40% nodulation, resulting in stubble soybean photosynthetic nitrogen fixation capacity was significantly reduced. The soybean root, litter and other by stimulating the growth of Penicillium, causing further harm to soybeans. The study on the regulation of solanin in continuous cropping soils showed that using biological control of soil actinomycetes MB could increase 8.4 ~ 18.9% of cropping soybean; biological control of marine actinomycete MB97 could make soybean replanting Increase production by 30.5%. Application of MB 97 formulations of continuous cropping soybean disease prevention, attenuated control of integrated control, so that crop five-year field trial of soybean yield equivalent soybean yield 4575kg · hm-2.