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目的 建立一快速、简便的血清伊曲康唑 (ITZ)药物浓度的反相高效液相色谱测定方法。方法 2 0 0 μl血清 ,用叔丁基甲醚一步提取后氮气吹干 ,复溶后直接进样分析。流动相为甲醇 0 .5 %醋酸铵 乙醚 (36 5∶110∶2 5 ) ;流速 0 .8ml·min-1;检测波长2 6 1nm ;SupelcosilLC18色谱柱 ,柱温 40℃ ;室温操作。结果 ITZ与内标在血清中分离良好 ,tR 分别为 8.3和 10min ;10～ 5 0 0ng·ml-1浓度范围线性良好 (r=0 .9997,n =5 ) ,富集进样方法可检出浓度达 2ng·ml-1;样品操作 2 0min内可完成。结论 本方法取样量小 ,操作快速简便 ,灵敏度高 ;适用于治疗药物监测和临床药物动力学和药效学研究。
Objective To establish a rapid and simple method for the determination of the concentration of itraconazole (ITZ) in serum by reversed-phase high performance liquid chromatography. Methods 200 μl of serum was extracted with tert-butyl methyl ether and dried in nitrogen. The sample was directly injected and analyzed after reconstitution. The mobile phase was methanol 0.5% ammonium acetate and ethyl ether (36 5:110:25); the flow rate was 0.8ml · min-1; the detection wavelength was 261nm; the Supelcosil LC18 column was operated at room temperature. Results ITZ and internal standard were well separated in serum with tR of 8.3 and 10 min, respectively. The concentration range of 10 ~ 500 ng · ml-1 was linear (r = 0.9997, n = 5) The concentration of 2ng · ml-1; sample operation can be completed within 20min. Conclusion The method of sampling small, fast and easy operation, high sensitivity; suitable for the treatment of drug monitoring and clinical pharmacokinetics and pharmacodynamics.