论文部分内容阅读
目的:探讨唾液腺腺样囊性癌高低转移细胞株(ACC-M、ACC-2)裸鼠移植瘤中基质金属蛋白酶(matrixmetalloproteinases,MMPs)及基质金属蛋白酶组织抑制剂(tissueinhibitorofmetalloproteinases,TIMPs)的表达。方法:采用ACC-M、ACC-2细胞株,建立ACC-M、ACC-2细胞株的裸鼠皮下移植瘤模型。在2组模型中,每组随机取3只裸鼠,用半定量RT-PCR分析移植瘤中MMP-2、MMP-7、MMP-9、TIMP-1、TIMP-2基因的表达水平,同时用免疫组化方法检测2个细胞株裸鼠移植瘤的MMP-2、MMP-7、MMP-9、TIMP-1、TIMP-2蛋白的表达情况,检测并记录其积分光密度值。所有检测数据用SPSS11.5软件进行均数t检验处理。结果:半定量RT-PCR和免疫组化结果均显示,MMP-2、MMP-7、MMP-9、TIMP-1的表达为ACC-M显著高于ACC-2(P<0.05);TIMP-2在2个细胞株裸鼠移植瘤的表达差异无统计学意义(P>0.05)。结论:MMP-2、MMP-7、MMP-9、TIMP-1的表达差异可能与ACC-M、ACC-2转移能力的大小相关。
Objective: To investigate the expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in xenografted salivary adenoid cystic carcinoma cell lines (ACC-M, ACC-2) Methods: ACC-M and ACC-2 cell lines were used to establish subcutaneous xenograft models of ACC-2 and ACC-2 cell lines in nude mice. Three nude mice were randomly selected from each of two groups. The expression of MMP-2, MMP-7, MMP-9, TIMP-1 and TIMP-2 was detected by semi-quantitative RT- The expression of MMP-2, MMP-7, MMP-9, TIMP-1 and TIMP-2 in the two cell lines was detected by immunohistochemistry. The integral optical density was measured and recorded. All test data using SPSS11.5 software for t test. Results: The results of semi-quantitative RT-PCR and immunohistochemistry showed that the expression of MMP-2, MMP-7, MMP-9 and TIMP-1 were significantly higher in ACC-M than in ACC- 2 in two cell lines nude mice xenograft tumor no significant difference (P> 0.05). Conclusion: The differences in the expression of MMP-2, MMP-7, MMP-9 and TIMP-1 may be related to the metastatic capacity of ACC-M and ACC-2.