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为了建立一种磁性纳米Fe3O4颗粒携载聚合阿霉素的制备方法,探讨载药纳米颗粒对K562及其耐药株K562/A02的作用,用机械吸附聚合法在4℃、37℃下以不同药球比聚合24、48小时合成载药纳米颗粒,用噻唑蓝比色法检测细胞与载药纳米颗粒悬液孵育48小时后的生存率,并计算细胞抑制率。结果表明:随着磁性纳米Fe3O4颗粒浓度增加,两种细胞抑制率均提高,聚合在4℃、48小时时的细胞抑制率分别高于37℃、24小时的细胞抑制率。结论:磁性纳米Fe3O4颗粒可通过机械吸附法携载阿霉素,聚合具有温度、时间依赖特性;载药纳米颗粒有逆转多药耐药作用。
In order to establish a preparation method of magnetic nano-Fe3O4 particles carrying polymeric doxorubicin, the effect of drug-loaded nano-particles on K562 and its resistant strain K562 / A02 was explored by mechanical adsorption polymerization at 4 ℃ and 37 ℃ The drug-loaded nanoparticles were synthesized at 24 and 48 hours after polymerization. The survival rate of the cells incubated with drug-loaded nanoparticles suspension for 48 hours was measured by thiazolyl blue colorimetric assay, and the cell inhibition rate was calculated. The results showed that with the increase of magnetic nano-Fe3O4 particle concentration, the inhibitory rates of the two kinds of cells increased. The cell inhibitory rates at 4 and 48 hours of polymerization were respectively higher than those at 37 and 24 hours. Conclusion: Magnetic nano-Fe3O4 particles can carry doxorubicin by mechanical adsorption. The polymerization has temperature-dependent and time-dependent characteristics. Drug-loaded nano-particles can reverse multidrug resistance.