论文部分内容阅读
目的了解该地区莱姆病传播媒介蜱地理分布及其病原菌携带情况与人群、动物莱姆病感染率。方法应用BSK、直接免疫荧光抗体法(DFA)进行病原体培养、检测。应用酶联免疫吸附试验(ELISA)进行人群、动物IgG抗体检测。应用间接免疫荧光抗体法(IFA)对分离到的伯氏疏螺旋体菌株进行鉴定。应用rrf(23s)间隔区扩增的限制型内切酶,对培养出的流行菌株进行致病性基因分型。结果媒介蜱带菌率为39.22%(551/1405)、人群平均感染率为6.99%(70/1002)、四种动物感染率为27.15%(177/652)。基因分型至少存在2种基因型B.garinii和B.afzelii。结论延边、吉林地区存在莱姆病疫原地,人群、动物感染率较高。
Objective To understand the geographical distribution of tick-borne Lyme disease vectors and the pathogen-carrying status and population, animal Lyme disease infection rates in the area. Methods BSK, direct immunofluorescence antibody method (DFA) for pathogen culture, detection. Enzyme-linked immunosorbent assay (ELISA) was used to detect IgG antibodies in human and animals. The isolated Borrelia burgdorferi strain was identified by indirect immunofluorescence antibody assay (IFA). Pathogenic genotyping was performed on the developed strains using restriction endonucleases amplified from the rrf (23s) spacer. Results The rate of borne ticks was 39.22% (551/1405), the average rate of infection was 6.99% (70/1002), and the infection rate of four animals was 27.15% (177/652). Genotyping There are at least two genotypes, B.garinii and B.afzelii. Conclusions Lyme disease epidemic exists in Yanbian and Jilin areas, and population and animal infection rates are high.