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人参(Panax ginseng)的水提取物具有与胰岛素相似的、能显著抑制离体脂肪细胞肾上腺素诱导的脂解活性。作者从其中分离出其活性物质(PⅡ),并证明此活性物质为14个氨基酸组成的多肽。方法:将人参干粉的水提物对水透析,透出物以DEAE-纤维粉分批吸附,再用1MNH_4HCO_3 pH8缓冲液洗脱。洗脱液用Sephadex G-10脱盐,再经Se-phadex G-50层析,用水洗脱,即可收集活性部位,浓缩后调至pH5,过夜,取上清液经Avicel纤维粉柱层析,用正丁醇-乙酸-水(3:1:1)混合液洗脱得三个峰。将峰Ⅱ再同样层析两次,并经Sephadex G-10柱脱盐,洗
The water extract of Panax ginseng has insulin-like properties and significantly inhibits epinephrine-induced lipolytic activity of ex vivo adipocytes. The authors isolated their active substance (PII) from it and proved that the active substance is a polypeptide of 14 amino acids. Methods: The aqueous extract of ginseng powder was dialyzed against water. The effluent was fractionated by DEAE-fiber powder and then eluted with 1 M NH_4HCO_3 pH8 buffer. The eluate was desalted with Sephadex G-10, and then by Se-phadex G-50 chromatography, eluted with water to collect the active site, concentrated to pH5 overnight and the supernatant was subjected to Avicel fiber column chromatography , With n-butanol - acetic acid - water (3: 1: 1) mixture of three peaks. Peak II was again chromatographed twice more and desalted by Sephadex G-10 column